產品名稱：NT-3 Rat ELISA Kit
- 產品編號
- BEK1174
- 產品規格
- 96T
- 價格
- 2800.00
- 來源
- -
- 交叉反應
- Rat
- 免 疫 原
- -
- 產品簡介
- This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-NT-3 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-NT-3 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NT-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NT-3 can be calculated.
Kit components
1. One 96-well plate pre-coated with anti-Rat NT-3 antibody
2. Lyophilized Rat NT-3 standards: 2 tubes (10 ng / tube)
3. Sample / Standard diluent buffer: 30ml
4. Biotin conjugated anti-Rat NT-3 antibody (Concentrated): 130μl. Dilution: 1:100
5. Antibody diluent buffer: 12ml
6. Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated): 130μl. Dilution: 1:100
7. ABC diluent buffer: 12ml
8. TMB substrate: 10ml
9. Stop solution: 10ml
10. Wash buffer (25X): 30ml
- 產品別名
- -
- 產品成分
- -
- 檢測范圍
- 15.6 pg/ml-1000 pg/ml
- 檢測樣品
- For quantitative detection of NT-3 in rat serum, body fluids, tissue lysates or cell culture supernatants.
- 產品應用
- ELISA
- 保存條件
- 2-8℃6個月 -20℃一年
- 背景資料
- Neurotrophin-3 is a neurotrophic factor in the NGF (Nerve Growth Factor) family of neurotrophins. It is encoded by the NTF3 gene, which localized to 12p13. It is a protein growth factor which has activity on certain neurons of the peripheral and central nervous system; it helps to support the survival and differentiation of existing neurons, and encourages the growth and differentiation of new neurons and synapses. Ramer et al. (2000) concluded that neurotrophic factor treatment may serve as a viable treatment in promoting recovery from root avulsion injuries. Although the vast majority of neurons in the mammalian brain are formed prenatally, parts of the adult brain retain the ability to grow new neurons from neural stem cells; a process known as neurogenesis.
- 參考資料
- 1. Maisonpierre PC, Le Beau MM, Espinosa R 3rd, Ip NY, Belluscio L, de la Monte SM, Squinto S, Furth ME, Yancopoulos GD (Oct 1991). "Human and rat brain-derived neurotrophic factor and neurotrophin-3: gene structures, distributions, and chromosomal localizations". Genomics 10 (3): 558–68. 2. Ramer, M. S., Priestley, J. V., McMahon, S. B. Functional regeneration of sensory axons into the adult spinal cord. Nature 403: 312-316, 2000.
